Part I Theory and General Techniques
Chapter 1 Introduction
Chapter 2 General Laboratory Procedures
Chapter 3 Extraction, Purification, and Concentration of Biomolecules
Chapter 4 Purification and Identification of Biomolecules by Chromatography
Chapter 5 Characterization of Biornolecules by Electrophoresis
Part II Experiments
Experiment 1 Properties of Proteins
Experiment 2 Quantitative Determination of Protein
Experiment 3 Extraction and Purification of Tyrosinase from Potato and Determination of the Enzyme Activity
Experiment 4 Determination of the Michaelis Constant for Tyrosinase and Effects that Affect the Activity
Experiment 5 Staining and Identification of Active Superoxide Dismutase
Experiment 6 Preparation of E.Coli Competent Cells and Introduction DNA into Host Cells
Experiment 7 Extraction and Quantitative Analysis of Plasmid DNA via Alkaline Lysis Method
Experiment 8 Identification of Transformation Products via Polymerase Chain Reaction and Restriction Endonuclease Digestion
Experiment 9 Extraction and Identification of Total RNA from Animal Tissues and Cells
Experiment 10 Reverse Transcription-polymerase Chain Reaction
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